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Reproducibility of electron microscope magnifications with digital display of individual lens currents
Author(s) -
Dunn Robert F.,
Preiss Guenther W. B.
Publication year - 1974
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1974.tb03957.x
Subject(s) - magnification , projector , optics , lens (geology) , calibration , microscope , reproducibility , focal length , materials science , physics , chemistry , chromatography , quantum mechanics
SUMMARY Quantitative measurements on electron micrographs with less than 10% error require precise calibration of all lenses contributing to the final image magnification. Calibration of the magnification to approximately 1.5% is easily attained by using simple statistical methods and a digital display milliampere meter capable of measuring the objective, intermediate and projector lens currents independently. The method detailed, readily adaptable to other electron microscopes, requires the projector lens current be held constant, with the magnification determined by varying the intermediate lens current and corrections made on the basis of calibrated objective lens current effect. The errors of magnification, approximately 1.5%, are the same range as the test grid variation which appears to be the limiting factor. Even with this limitation, magnifications have been determined reproducible to within less than 0.2% in daily use over a 6 month period.