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Staining of biological material for the scanning electron microscope
Author(s) -
Carr K. E.,
McGadey J.
Publication year - 1974
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1974.tb03944.x
Subject(s) - scanning electron microscope , electron microscope , biological specimen , secondary electrons , staining , scanning confocal electron microscopy , materials science , electron , acceleration voltage , environmental scanning electron microscope , conventional transmission electron microscope , coating , negative stain , signal (programming language) , chemistry , biophysics , optics , nanotechnology , scanning transmission electron microscopy , biology , pathology , physics , computer science , composite material , medicine , quantum mechanics , cathode ray , programming language
SUMMARY A histochemical technique has been used in scanning electron microscopy to localize specific areas in biological material. The specific contrast introduced by the staining procedure depends on the interaction of complex variables such as the coating material, the accelerating voltage and the use of a primary or secondary electron signal.