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Current developments in high voltage electron microscopy
Author(s) -
Cosslett V. E.
Publication year - 1974
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1974.tb03936.x
Subject(s) - voltage , high voltage , materials science , ionization , nanotechnology , engineering physics , low voltage , biological specimen , nuclear engineering , optoelectronics , optics , electrical engineering , chemistry , physics , engineering , ion , organic chemistry
SUMMARY In respect of instrument design two main developments are taking place in high voltage electron microscopy: towards even higher operating voltages (3–5 MV) and towards higher resolving power at moderate voltages (250–600 kV). Applications of existing instruments (650 kV‐1.2 MV) are still primarily in metallurgy, especially for radiation damage studies, but their usefulness for biological research is now being actively explored. Microchambers have been developed for observing specimens, both metallurgical and biological, in a controlled gaseous or liquid environment. The prospects for observing living material, except at low magnifications, remain very doubtful on account of ionization effects, but informative work with wet specimens should be possible.