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Detection of haemoglobin in red cells of Xenopus laevis by immunofluorescent double labelling
Author(s) -
Jurd Richard D.,
Maclean Norman
Publication year - 1974
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1974.tb03930.x
Subject(s) - xenopus , tadpole (physics) , metamorphosis , fluorescein , biology , fluorescein isothiocyanate , antibody , microbiology and biotechnology , guinea pig , red cell , fluorescence , biochemistry , immunology , endocrinology , medicine , larva , botany , gene , physics , particle physics , quantum mechanics
SUMMARY Red cells from metamorphosing Xenopus laevis were treated with a fluorescein‐conjugated guinea‐pig antibody to Xenopus tadpole haemoglobin, a rabbit antibody to Xenopus adult haemoglobin, and a rhodamine‐conjugated goat anti‐rabbit globulin antibody. The presence of both red and green fluorescence in some cells indicated that at metamorphosis a proportion of the red cells contained both adult and tadpole haemoglobins.