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The etching of thick Araldite‐embedded sections for scanning electron microscopy
Author(s) -
Thurley Kenneth W.,
Mouel Walter C.
Publication year - 1974
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1974.tb03887.x
Subject(s) - araldite , scanning electron microscope , distilled water , transmission electron microscopy , materials science , epoxy , electron microscope , etching (microfabrication) , composite material , optics , chemistry , chromatography , nanotechnology , adhesive , layer (electronics) , physics
SUMMARY A rapid method of correlation of information from the images of the scanning electron microscope, the transmission electron microscope and the light microscope, from biological tissues embedded in epoxy‐resin. It allows a perception in depth at the SEM level of areas already defined by the other two modes. One micrometre sections of Araldite‐embedded tissues are cut, dried onto glass cover slips, and the embedding medium etched away, with a saturated solution of NaOH in absolute ethanol. After washing in absolute ethanol and distilled water the resultant osmicated ‘skeleton’ is coated and viewed in the SEM.