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The use of X‐ray microanalysis to investigate problems encountered in enzyme cytochemistry *
Author(s) -
Ryder T. A.,
Bowen I. D.
Publication year - 1974
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1974.tb03877.x
Subject(s) - cytochemistry , microanalysis , electron probe microanalysis , chemistry , staining , enzyme , biochemistry , mineralogy , electron microprobe , biology , organic chemistry , genetics
SUMMARY The nature of the reaction product and non‐specific staining obtained using lead salt methods, for localizing phosphatase activities, has been determined using energy dispersive X‐ray microanalysis. The detectability of ‘non‐electron dense’ elements has permitted the development of an alternative azo dye method involving the coupling of an enzymatically released, insoluble primary reaction product with the active diazotate of 2, 5‐dichloroaniline (Fast Scarlet GG). The resultant azo dye was detected in ultrathin cryosections. The localization achieved by this method and the advantages of combining ultracryotomy and X‐ray microanalysis with enzyme cytochemistry is discussed.