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A comparative survey of techniques for preparing plant surfaces for the scanning electron microscope
Author(s) -
Parsons Elizabeth,
Bole Barbara,
Hall D. J.,
Thomas W. D. E.
Publication year - 1974
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1974.tb03867.x
Subject(s) - scanning electron microscope , materials science , environmental scanning electron microscope , microscope , petal , electron microscope , coating , nanotechnology , optics , composite material , botany , biology , physics
SUMMARY A comparative investigation of techniques for the preparation of soft botanical tissue for the scanning electron microscope has been carried out using the leaves and petals of Pelargonium zonale as test specimens. Twelve different preparative procedures involving combinations of fixation, dehydration, air drying, freeze drying, critical point drying, coating methods, replicas and a temperature controlled specimen stage were tested.