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Localization of acid and alkaline phosphatase
Author(s) -
Bauer Heinz,
Sigarlakie Elsje
Publication year - 1973
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1973.tb04673.x
Subject(s) - alkaline phosphatase , acid phosphatase , cytoplasm , vesicle , vacuole , endoplasmic reticulum , glutaraldehyde , biochemistry , phosphatase , chemistry , cell wall , budding , organelle , yeast , microbiology and biotechnology , biology , biophysics , enzyme , membrane , chromatography
SUMMARY Ultrathin frozen sections of glutaraldehyde fixed yeast cells have been successfully used for the demonstration of acid and alkaline phosphatase. Acid phosphatase was localized over the cell wall of both the mother cell and the bud as well as over the newly forming cross wall (septum). Cytoplasmic vesicles (vacuoles, lysosomes?) located close to the cell wall showed a positive reaction for acid phosphatase as well. After 3 h glutaraldehyde fixation an activation of the nuclear acid phosphatase was observed. Lead precipitates were predominantly found over the nucleolar material of ‘resting’ and budding cells. Alkaline phosphatase could be demonstrated in the ‘yeast‐mesosome’ and within the plasmalemma invaginations. After separation of the bud, small vesicles, probably derived from the endoplasmic reticulum showed a strong positive reaction for alkaline phosphatase. In frozen sections incubated for alkaline phosphatase, non‐specific lead precipitates were found in the nucleus and along the plasmalemma invaginations.