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Processing small delicate biological specimens for scanning electron microscopy
Author(s) -
Marchant Harvey J.
Publication year - 1973
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1973.tb03792.x
Subject(s) - scanning electron microscope , materials science , nanotechnology , microscopy , electron microscope , chemistry , optics , composite material , physics
SUMMARY To overcome the problems of collecting and handling delicate cells while dehydrating them for scanning electron microscopy, a method is suggested whereby cells are collected on solvent resistant Millipore filters. The schedule used for successfully processing a wide range of algae, protozoa and some other organisms is outlined as is the construction of a simple carrier to hold the filter in the critical point drying apparatus.