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A technique for the cytochemical demonstration in the electron microscope of glucose‐6‐phosphate dehydrogenase activity in erythrocytes of malaria‐infected animals
Author(s) -
Theakston R. D. G.,
Fletcher K. A.
Publication year - 1973
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1973.tb03786.x
Subject(s) - electron microscope , vacuole , formazan , malaria , dehydrogenase , biochemistry , chemistry , biology , glucose 6 phosphate dehydrogenase , plasmodium (life cycle) , enzyme , parasite hosting , immunology , optics , cytoplasm , physics , world wide web , computer science
SUMMARY A technique for the cytochemical demonstration in the electron microscope of glucose‐6‐phosphate dehydrogenase activity in malaria‐infected erythrocytes is described. Reaction product occurred as diffuse electron‐dense deposits of the formazan of MTT in the red cells. No activity was observed within the malaria parasites themselves, except in the contents of food vacuoles. The results of a series of control experiments are also described.