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A method of freeze‐drying and fixing spermatazoa for electron microscopy
Author(s) -
Johnston H. S.,
Reid O.
Publication year - 1972
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1972.tb03754.x
Subject(s) - fixative , glutaraldehyde , electron microscope , semen , electron micrographs , chromatography , chemistry , microscopy , biology , pathology , anatomy , biochemistry , cytoplasm , optics , medicine , physics
SUMMARY A procedure is described whereby freshly ejaculated bull semen is freeze‐dried, then treated by glutaraldehyde fixative and processed for electron microscope observation of the spermatozoa. Some of the structural features of bull spermatozoa are demonstrated in electron micrographs. In general the procedure may be found useful as an alternative technique for the examination of such cell‐carrying fluids as whole blood, cerebrospinal fluid, as well as bacterial cultures.