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The use of a low‐viscosity epoxy resin in the preparation of undecalcified bone sections for light microscopy
Author(s) -
Xipell J. M.,
Gladwin R. C.
Publication year - 1972
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1972.tb03753.x
Subject(s) - epoxy , materials science , microscopy , viscosity , cancellous bone , von kossa stain , optical microscope , composite material , biomedical engineering , polarized light microscopy , chemistry , optics , scanning electron microscope , anatomy , pathology , medicine , physics , biochemistry , alkaline phosphatase , enzyme
SUMMARY A method using the Spurr low‐viscosity epoxy resin medium for the preparation of Von Kossa impregnated undecalcified bone sections for light microscopy is described. The method gives high quality thin sections (0·6–1·0 μm) of cancellous bone and overcomes some of the difficulties experienced with other plastics. The procedure is relatively simple and is well suited for use in a routine diagnostic laboratory.