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Preliminary studies of the effects of DMSO on histochemical reactions
Author(s) -
Ward P.,
Smith Audrey U.
Publication year - 1971
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1971.tb03697.x
Subject(s) - liquid nitrogen , enzyme , staining , reagent , chemistry , dimethyl sulfoxide , mitochondrion , biochemistry , kidney , biophysics , biology , organic chemistry , endocrinology , genetics
SUMMARY Slices of mammalian kidney were treated with concentrations of dimethyl sulphoxide (DMSO) theoretically high enough to protect living cells against damage during freezing and thawing. The blocks were cooled slowly to −45°C and then quenched in liquid nitrogen. Sections were cut at −45°C and post‐fixed in various reagents. They were stained for lysosomal and mitochondrial enzymes. Cellular and tissue morphology was better preserved than in tissue which had been treated by conventional histochemical freezing methods. The staining of the enzymes was satisfactory and the localization of mitochondrial enzymes, in particular, was sharper.