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An improved apparatus for perfusion fixation with automatic pressure control
Author(s) -
Gil Joan,
Weibel Ewald R.
Publication year - 1971
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1971.tb02372.x
Subject(s) - fixation (population genetics) , perfusion , hydrostatic pressure , peristaltic pump , biomedical engineering , oncotic pressure , peristalsis , materials science , chemistry , medicine , mechanics , cardiology , physics , mechanical engineering , engineering , biochemistry , albumin , gene
Summary A method and equipment are described which allows vascular‐perfusion fixation for electron microscopy under controlled pressure conditions. Hydrostatic pressure can be kept constant during fixation by automatic adjustment of the flow rate of a peristaltic pump which is governed by a fully automatic remote‐control device. Colloid osmotic pressure of the perfusates must also be adjusted to assure adequate balance of the pressures in the tissue at physiological levels.