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Dark‐field electron microscopy of unstained and unshadowed DNA *
Author(s) -
Haydon Glen B.,
Lemons Ross A.,
Clayton David A.
Publication year - 1971
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1971.tb02362.x
Subject(s) - dark field microscopy , electron microscope , microscopy , optics , electron , field (mathematics) , materials science , biological specimen , chemistry , physics , nuclear physics , mathematics , pure mathematics
Summary The vastly increased contrast made possible by tilted‐beam dark‐field electron microscopy enables strands of DNA prepared by the Kleinschmidt technique to be clearly imaged without the heavy‐metal staining or shadowing usually required. This technique is applicable over a wide range of magnifications and is easily accomplished with several commercial instruments.