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A high resolution technique for the fine‐structural localization of acid hydrolases
Author(s) -
Bowen I. D.
Publication year - 1971
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1971.tb02358.x
Subject(s) - nitrobenzene , acid phosphatase , chemistry , enzyme , resolution (logic) , biochemistry , chromatography , catalysis , artificial intelligence , computer science
Summary An azo dye technique is described for the fine‐structural localization of acid hydrolases. Enzymatically released primary reaction product, an insoluble substituted naphthol, is coupled with ρ‐nitrobenzene diazonium‐tetrafluoborate to produce a dense particulate azo dye. Simultaneous and post‐coupling procedures are employed. The method is potentially applicable to all acid hydrolases and is free from the artifacts which beset the commonly used lead salt methods. The localization of β‐glucuronidase and acid phosphatase in rat and slug liver is specifically dealt with here. Lysosomal and cytoplasmic sources of enzyme activity are revealed.