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Preparation of human skin for high‐resolution scanning electron microscopy using phosphate buffered crude bacterial α‐amylase
Author(s) -
Finlay J. B.,
Hunter J. A. A.,
Steven F. S.
Publication year - 1971
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1971.tb02267.x
Subject(s) - amylase , resolution (logic) , phosphate buffered saline , electron microscope , scanning electron microscope , human skin , chemistry , microscopy , phosphate , chromatography , enzyme , buffer (optical fiber) , high resolution , biophysics , materials science , biochemistry , biology , optics , geology , telecommunications , physics , remote sensing , artificial intelligence , computer science , composite material , genetics
SUMMARY A technique for preparing human skin for high‐resolution scanning electron microscopy using phosphate‐buffered crude bacterial α‐amylase is described. Effects of the buffer solution alone are studied as well as the proteolytic activity of the enzyme solution used. The biochemical implications of these observations are discussed with regard to the in situ structure of the tissue.