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Preparation and mechanical requirements for freeze‐etching thick‐walled plant tissues *
Author(s) -
Robards A. W.,
Parish G. R.
Publication year - 1971
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1971.tb02265.x
Subject(s) - etching (microfabrication) , materials science , process (computing) , plant tissue , nanotechnology , computer science , biology , botany , layer (electronics) , operating system
SUMMARY The freeze‐etching technique is useful in studying cells from higher plant tissues even when thick cell walls are present. However, such cells require special consideration both in preparation for the freeze‐etching process as well as during fracturing and subsequent stages. Modifications to the technique and to the apparatus are described which make it possible to freeze‐etch thick‐walled plant tissues more successfully than by employing other methods.