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A technique for studying one and the same section of a cell in sequence with the light and electron microscope
Author(s) -
Wells B.,
Cour L. F. La
Publication year - 1971
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1971.tb02262.x
Subject(s) - electron microscope , microscope , optics , materials science , stain , scanning electron microscope , staining , chemistry , biology , physics , genetics
SUMMARY Methods for mounting and staining relatively thin sections on electron microscope grids, in order that one and the same section of a cell can be photographed in sequence with the light and electron microscope are described. Toluidine blue is used as a stain and hexachlorabuta‐1,3 diene as a medium which enables the grid carrying the stained sections to be temporarily mounted under a coverslip and examined with an oil‐immersion lens. Results obtained with pollen mother cells of Fritillaria lanceolata at zygotene are illustrated.