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Orientation of spherical specimens for ultrathin sectioning in selected planes by embedding in agar
Author(s) -
Scott Kay,
Tarin D.,
Sharp J. A.
Publication year - 1970
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1970.tb02227.x
Subject(s) - araldite , osmium tetroxide , agar , glutaraldehyde , materials science , orientation (vector space) , electron microscope , microscopy , optics , chemistry , chromatography , composite material , geometry , mathematics , biology , layer (electronics) , physics , adhesive , bacteria , genetics
SUMMARY The use of agar for supporting specimens in a desired orientation during processing for electron microscopy is described. The specimen is fixed in glutaraldehyde and orientated in liquid agar which is then allowed to set. The agar block is post‐fixed in osmium tetroxide and embedded in Araldite. Ultrathin sections are easily obtained and tissue preservation is satisfactory.