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Electron staining of synaptic vesicles using the Champy‐Maillet technique
Author(s) -
Iraldi Amanda Pellegrino,
Suburo Angela Maria
Publication year - 1970
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1970.tb02211.x
Subject(s) - iodide , chemistry , vesicle , iodine , osmium tetroxide , synaptic vesicle , matrix (chemical analysis) , biophysics , osmium , inorganic chemistry , chromatography , biochemistry , electron microscope , organic chemistry , ruthenium , biology , membrane , physics , optics , catalysis
SUMMARY Synaptic vesicles from rat pineal nerves can be stained by Champy‐Maillet mixtures containing osmium tetroxide and a soluble iodide. Two components of the vesicle (the matrix and the dense core) are revealed in a different way depending on at least two factors: the iodide employed and the final pH of the mixture. We have tried the following mixtures: zinc iodide at pH 5·5 (ZIO), cadmium iodide at pH 6·0 (CIO) and potassium iodide at pH 7·2 (KIO‐7) or pH 5·5 (KIO‐5). The matrix is more reactive with ZIO, while the dense core is more reactive with CIO and especially with KIO‐7. With the only difference of a lower pH, KIO‐5 stains the whole matrix of some vesicles and also reveals some tubular structures which are more frequently found in preterminal axons.