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Electron cytochemical localization of cystine in plant cell walls
Author(s) -
Thompson E.,
Colvin J. Ross
Publication year - 1970
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1970.tb02210.x
Subject(s) - cystine , electron microscope , metal , chemistry , keratin , crystallography , biochemistry , biology , organic chemistry , cysteine , optics , enzyme , paleontology , physics
SUMMARY A method for specific, cytochemical staining of cystine residues was applied to thin sections of human hair keratin, two yeasts, one fungus and one green alga, for observations in the electron microscope. The method, which deposits granules of metallic silver at or close to the site of cystine residues, may be a useful means of investigating the detailed distribution of disulphide bonds in cells provided that the following criteria are rigorously applied. First, grains of metallic silver must remain after exhaustive sodium thiosulphate extraction; second, the deposition of grains of metallic silver must be inhibited by prior alkylation of the disulphide linkage. Finally, the method should be used in conjunction with other available means of analysis.