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A new acridine orange method for staining nucleic acid and its application to germinating wheat seeds
Author(s) -
Price C. E.
Publication year - 1969
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1969.tb00695.x
Subject(s) - acridine orange , nucleic acid , germination , rna , staining , scutellum , embryo , orange (colour) , biochemistry , chemistry , biology , dna , microbiology and biotechnology , botany , food science , genetics , gene
SUMMARY A new method of nucleic acid staining based on acridine orange is described and applied to sections of wheat seed. The coleorhiza of the ungerminated seed has an RNA content as high as the embryonic leaves and roots. During the first 24 h of germination the RNA levels of the roots, leaves and scutellum remain relatively unchanged while that of the coleorhiza falls to a low level. The possibility is discussed that the rapidly‐mobilized RNA functions as a storage product which is hydrolysed and incorporated into fresh RNA in the adjacent embryo during germination.