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A stable iron‐hæmatoxylin solution for staining the chromatin of cell nuclei
Author(s) -
Slidders W.
Publication year - 1969
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1969.tb00694.x
Subject(s) - mordant , ferrous , staining , chemistry , stain , iodate , van gieson's stain , nuclear chemistry , inorganic chemistry , iodide , biology , genetics , organic chemistry , dyeing , h&e stain
SUMMARY By using ferrous sulphate as mordant, sodium iodate as oxidant and aluminium chloride as stabilizer, it proved possible to formulate a stable single‐solution iron‐hæmatoxylin. This provides a resistant nuclear stain, sufficiently acid‐fast to be suitable for counterstaining with van Gieson or the trichrome methods. This solution, ferrous hæmatein, can replace the celestin blue‐hæmalum sequence.