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A method of processing third‐stage larvae of the nematode Trichostrongylus colubriformis for electron microscopy
Author(s) -
Smith K.
Publication year - 1969
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1969.tb00675.x
Subject(s) - electron microscope , microscopy , fixation (population genetics) , distortion (music) , materials science , scanning electron microscope , nematode , biology , optics , composite material , optoelectronics , ecology , physics , biochemistry , amplifier , cmos , gene
SUMMARY A method for processing third‐stage larvæ for electron microscopy is described. Using simple apparatus larvæ are separated from fæcal material by migration to a warmed area; they are then immobilized and chopped up in agar prior to fixation. No cellular distortion is observed in ultra‐thin sections.