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A rapid methylene blue‐basic fuchsin stain for semi‐thin sections of peripheral nerve and other tissues
Author(s) -
Aparicio S. R.,
Marsden P.
Publication year - 1969
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1969.tb00659.x
Subject(s) - methylene blue , staining , toluidine , alizarin red , chemistry , pathology , stain , differential staining , guinea pig , anatomy , microbiology and biotechnology , biology , medicine , biochemistry , photocatalysis , endocrinology , catalysis
SUMMARY Differential histological staining of various tissues is achieved in epon, araldite or glycol methacrylate sections 0·25–0·5 μ thick, from human, guinea‐pig and rat origin. The staining procedure is as follows: 1% methylene blue in 1% borax 15–30 sec at 70°C; wash in hot water; 2% basic fuchsin in distilled water 1–2 min at room temperature; wash and mount in DPX. Results : with all tissues: nuclei pale violet, cytoplasm blue. Special histological components: collagen red, myelin deep blue, elastin deep violet, axoplasm pale blue. Although of general utility the method is especially useful for peripheral nerve and blood vessels.