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Electron phase and amplitude images of stained biological thin sections
Author(s) -
Haydon Glen B.
Publication year - 1969
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1969.tb00651.x
Subject(s) - amplitude , phase (matter) , resolution (logic) , materials science , electron microscope , biological specimen , optics , stain , scanning electron microscope , thin film , microscopy , chemistry , physics , staining , nanotechnology , composite material , computer science , biology , organic chemistry , artificial intelligence , genetics
SUMMARY Very‐high‐resolution electron microscopy demonstrates similar phase image ‘particles’ in uncontaminated, unsupported thin sections of epoxy‐embedded biological material and in simple thin carbon films, both of which show better than 5 Å resolution. Under such conditions it is possible to examine biological specimens for amplitude images well below the 20 Å limit commonly accepted for such preparations. With this very high resolution discrete amplitude images are found in sections after treatment with heavy‐metal stain. These amplitude images are as small as 10 Å, a size equal to that of lead aggregates, which they may represent.