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Storage of frozen materials for cryostat sectioning and “soluble‐compound” autoradiography
Author(s) -
APPLETON T. C.
Publication year - 1967
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1967.tb04528.x
Subject(s) - cryostat , liquid nitrogen , materials science , contamination , vitrification , radiochemistry , gamma irradiation , irradiation , chemistry , superconductivity , organic chemistry , quantum mechanics , andrology , biology , medicine , ecology , physics , nuclear physics
SYNOPSIS A technique is described for the storage of biological materials in air tight sachets made from a new aluminium‐polythene laminate. Tissues containing highly mobile soluble radioactive material have been stored for periods up to six months in these sachets which are stored in a liquid nitrogen refrigerator. This method of storage preserves the tissues in such a way as to prevent ice crystal damage, and also prevents cross‐contamination of radioactive material which would result from contact between radioactive specimens.