Premium
Changes in the fine structure of the mouse pancreatic acinar cell after fixation by chromium trioxide
Author(s) -
MCCRAE J. M.
Publication year - 1967
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1967.tb04523.x
Subject(s) - golgi apparatus , endoplasmic reticulum , chromium trioxide , chemistry , nuclear membrane , cytoplasm , biophysics , nucleolus , electron microscope , nucleus , anatomy , pathology , microbiology and biotechnology , biology , chromium , biochemistry , medicine , physics , optics , organic chemistry
SYNOPSIS The exocrine pancreatic cell of the mouse was the test object for the investigation. Tissue fixed with a 0.5 p.c. aqueous solution of chromium trioxide was embedded in Araldite and sections were examined with the electron microscope. Chromium trioxide does not convert the cytoplasm into a coagulum. What appears to be a coagulum in optical microscopy is in fact a three‐dimensional network of ergastoplasm (endoplasmic reticulum), the cisterna of which retain their identity (though the ribosomes are not preserved). The mitochondria are not destroyed, but no internal structure is seen. No vestige of the Golgi element remains. The inner nuclear membrane is preserved, the outer only here and there. Nothing that could be interpreted as a thread‐like structure is seen in the nucleus. The nucleolus appears homogeneous.