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Combined protein and DNA measurements in plant cells using the dinitrofluorobenzene and Feulgen techniques
Author(s) -
MITCHELL J. P.
Publication year - 1967
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1967.tb04517.x
Subject(s) - feulgen stain , optical density , dna , fixation (population genetics) , cell , chemistry , staining , biophysics , microbiology and biotechnology , biochemistry , biology , optics , physics , genetics , gene
SYNOPSIS The dinitrofluorobenzene reaction without subsequent chemical treatment has been shown to be suitable for combination with the Feulgen procedure to provide a means of measuring protein and DNA in the same cell. The density of dinitrofluorobenzene colouration per cell is affected by the fixation procedure and by the composition and temperature of the DNFB solution but with all the treatments used, the same reproducible maximum optical density per cell was obtained by increasing the temperature at which the reaction was carried out. The Feulgen procedure has no effect on the optical density of cells previously treated with dinitrofluorobenzene and is not affected itself by this previous staining procedure.