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An examination of some factors affecting histological preservation in frozen sections of unfixed tissue
Author(s) -
SILCOX A. A.,
POULTER L. W.,
BITENSKY LUCILLE,
CHAYEN J.
Publication year - 1965
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1965.tb02156.x
Subject(s) - cabinet (room) , anatomy , biology , engineering , mechanical engineering
SYNOPSIS No ice deformity could be detected histologically when rat liver was chilled rapidly to the temperature of solid carbon dioxide and was sectioned with a chilled knife in a cabinet refrigerated to −30°C. Slower chilling produced extra‐cellular ice deformities while elevated temperatures of the cabinet or the knife produced distortion probably due to heating during cutting.