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A starch film method for the identification of catalase in histological sections
Author(s) -
HALE ARTHUR J.
Publication year - 1965
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1965.tb02131.x
Subject(s) - catalase , hydrogen peroxide , starch , chemistry , iodide , materials science , nuclear chemistry , biochemistry , inorganic chemistry , enzyme
SYNOPSIS A method for the identification and localization of catalase in histological sections is described. Sections are placed in contact with a thin film of starch gel which is immersed in hydrogen peroxide and then in a solution of potassium iodide. The starch film turns dark blue except where the hydrogen peroxide in it has been destroyed by catalase. At these points the film remains white.