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Enzyme histochemistry of developing human skin in tissue culture
Author(s) -
PULLAR PETER,
LIADSKY CHARLOTTE
Publication year - 1965
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1965.tb02130.x
Subject(s) - keratin , esterase , enzyme , epithelium , cysteine , acid phosphatase , in vivo , biology , human skin , alkaline phosphatase , biochemistry , immunohistochemistry , tissue culture , chemistry , in vitro , pathology , immunology , medicine , genetics
SYNOPSIS Human foetal skin can develop in tissue culture using a synthetic chemically defined medium. The development produces squamous type epithelium which subsequently forms keratin. The assumption of squamous morphology is associated with the appearance of a reaction for acid phosphatase, non‐specific esterase and cysteine desulphurase. These enzymes show a distinct intensification in the zone between the squamous and keratin layers. The findings suggest that the process in this system closely parallels that in vivo.