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Studies on the cellular uptake of ribonuclease
Author(s) -
CORMACK D. H.
Publication year - 1965
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1965.tb02128.x
Subject(s) - rnase p , ribonuclease , vacuole , cytoplasm , enzyme , biology , in vitro , biochemistry , amoeba proteus , fluorescein , amoeba (genus) , microbiology and biotechnology , fibroblast , chemistry , rna , fluorescence , physics , quantum mechanics , gene
SYNOPSIS The uptake of RNase by two types of amoeba and a selection of vertebrate cells has been investigated using the fluorescein‐labelled enzyme. This method is considered satisfactory for the study since the fluorescein label migrated together with the enzyme during electrophoresis, and the labelled RNase retained one half of its enzymatic activity. Although RNase was ingested as droplets by both Amoeba proteus and Acanthamoeba sp., these two amoebae differed in their extent of surface adsorption of the enzyme and in the effect of RNase on the activity of their contractile vacuoles. Cytoplasmic droplets were also ingested in vitro by a small proportion of ascites cells, but there was no detectable uptake by rabbit kidney, mouse fibroblast, chick embryo or HeLa cultures.