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THE FALGIC ACID DYES AND THEIR APPLICATIONS IN HISTOLOGICAL STAINING
Author(s) -
Gurr E.,
MacConaill M. A.
Publication year - 1961
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1961.tb05228.x
Subject(s) - staining , stain , chemistry , acid dye , alizarin red , photochemistry , nuclear chemistry , art , organic chemistry , dyeing , biology , genetics
SYNOPSIS Sections stained with acid fuchsin and counterstained with light green SF (yellowish) show a striking polychrome effect in shades of red, violet, and blue. This is noteworthy for at least two reasons:(a) Blue is, of course, a primary colour and cannot, therefore, be produced by the mere admixture of red and green, which are the colours of the two dyes used. Chemical union must therefore take place between these two acid dyes to produce a series of new dyes, one of which is blue. (b) It has long been known to histologists that a basic dye will unite chemically with an acid dye to form a two‐colour stain, but similar union between two acid dyes was not hitherto thought possible or even considered.