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OPTICS OF THE OBJECT SPACE IN MICROSCOPY *
Author(s) -
Spinell B. M.,
Loveland R. P.
Publication year - 1959
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1959.tb04454.x
Subject(s) - optics , image quality , refractive index , computer science , observer (physics) , mathematics , image (mathematics) , algorithm , computer vision , physics , quantum mechanics
SYNOPSIS Change of the tube length or of the separation of the elements of the objective can be used to eliminate the image degradation caused by incorrect refractive index or thickness in the object space. These can be in either the cover‐slip or the immersion oil. The quantitative relations were studied. The method of compensation is effective even over an extreme range. A simple formulation was found to express the toleration of an observer to image degradation due to variation of the aforementioned factors from the designed optimum. This tolerance, expressed as*** + T(Δx) is affected only by the numerical aperture of the objective; T(Δx) = k(N.A.) −v . The two parameters, v and k , are significant, the latter being a measure of the strictness of the quality criterion. Graphs are presented from which these tolerances can be read.