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B. —QUANTITATIVE ELECTRON STAINING OF VIRUS PARTICLES
Author(s) -
Valentine R. C.
Publication year - 1958
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1958.tb02033.x
Subject(s) - electron micrographs , electron , staining , optics , scattering , negative stain , physics , electron microscope , constant (computer programming) , chemistry , biology , computer science , nuclear physics , genetics , programming language
SYNOPSIS As test objects for measuring the uptake of electron stains, virus particles have the advantage that their thickness is constant and known with fair accuracy. The fraction of the incident beam scattered by them out of the image can easily be deduced from the optical densities of electron micrographs and the degree of staining thus measured quantitatively. Some results are given and discussed in terms of electron scattering theory.