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V.—ELECTRON MIOROSCOPY AND MICRODISSECTION
Author(s) -
Bovey R.
Publication year - 1952
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1952.tb00993.x
Subject(s) - micromanipulator , collodion , electron microscope , materials science , microdissection , electron micrographs , microscope slide , microscope , environmental scanning electron microscope , optics , membrane , chemistry , computer science , physics , artificial intelligence , biochemistry , gene
A new technique is described for the preparation of biological specimens for electron microscope study, a de Fonbrune micromanipulator and an inverted microscope being used. The microdissection is made directly on the collodion film supported by a thin glass slide (cover‐slip) and afterwards the specimen is mounted on a grid. The method is illustrated with electron micrographs of nuclear and cell membranes, and its advantages are discussed.