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HISTOLOGICAL TECHNIQUES FOE THE ELECTRON MICROSCOPE
Author(s) -
Birbeck M. S. C.
Publication year - 1951
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1951.tb00395.x
Subject(s) - osmium tetroxide , microtome , electron microscope , wax , fixation (population genetics) , limiting , microscope , osmium , materials science , anatomy , chemistry , biomedical engineering , pathology , optics , biology , composite material , physics , medicine , biochemistry , engineering , mechanical engineering , ruthenium , gene , catalysis
SYNOPSIS. A technique for cutting thin sections for the electron microscope is described. Adequate fixation of the tissue is one of the limiting factors. The tissue is fixed in osmium tetroxide, embedded in Ester wax, and sectioned on a Cambridge rocking microtome. The technique has been used to examine various normal rat tissues.