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A CULTURE CHAMBER FOR THE MICROSCOPICAL STUDY OF LIVING BACTERIA WITH SOME OBSERVATIONS ON THE SPORE‐BEARING AEROBES
Author(s) -
Harris Nora K.,
Powell E. O.
Publication year - 1951
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1951.tb00394.x
Subject(s) - cellophane , spore , membrane , bacteria , bearing (navigation) , botany , biology , liquid medium , microbiology and biotechnology , chemistry , materials science , chromatography , composite material , biochemistry , physics , genetics , astronomy
SYNOPSIS. The culture chamber described consists essentially of a cellophane membrane on the upper surface of which micro‐organisms are grown. Their nutrient medium is supplied to the under surface of the membrane. The organisms can be studied microscopically by vertical illumination. The gaseous and liquid environment can be changed at will while a single cell or small group is kept under observation. Micromanipulative techniques can be applied. The use of the chamber is exemplified by experiments with members of the genus Bacillus .