Premium
SIMPLIFIED TECHNIQUES FOR THE EMPLOYMENT OF FLUORESCENCE MICROSCOPY
Author(s) -
King J.
Publication year - 1951
Publication title -
journal of the royal microscopical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0368-3974
DOI - 10.1111/j.1365-2818.1951.tb00388.x
Subject(s) - monochromator , fluorescence , condenser (optics) , optics , materials science , microscopy , fluorescence microscope , microscope , optical microscope , optoelectronics , light source , composite material , scanning electron microscope , wavelength , physics
SYNOPSIS. A method is described for using the compact‐source high‐pressure mercury‐vapour lamp for fluorescence microscopy in place of the conventional monochromator, with the aid of compound filters consisting of glass and liquid filters, giving bands of frequencies through a range from 2652 Å. to 3655 Å. “Perspex” or one of the new high ultra‐violet transmission glasses may be used for the condenser lenses and the ends of the liquid filters, in place of quartz. The adaptation of a reflecting objective, or a modification of the Chapman and Aldrich illuminator, each having aluminized surfaces, enables a concentrated beam of ultra‐violet light to be used with a fairly high magnification. A new range of fluorescent dyes known commercially as “optical bleaches” has been used for fluorescent staining of fibres and plant sections.