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Cell sheet engineering: a unique nanotechnology for scaffold‐free tissue reconstruction with clinical applications in regenerative medicine
Author(s) -
ElloumiHannachi I.,
Yamato M.,
Okano T.
Publication year - 2010
Publication title -
journal of internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.625
H-Index - 160
eISSN - 1365-2796
pISSN - 0954-6820
DOI - 10.1111/j.1365-2796.2009.02185.x
Subject(s) - tissue engineering , medicine , regenerative medicine , biomedical engineering , surface modification , clinical practice , scaffold , nanotechnology , cell , materials science , chemical engineering , chemistry , biochemistry , engineering , family medicine
. Cell sheet technology (CST) is based on the use of thermoresponsive polymers, poly(N‐isopropylacrylamide) (PIPAAm). The surface of PIPAAms is formulated in such a way as to make its typical thickness <100 nm. In this review, we first focus on how the methods of PIPAAm‐grafted surface preparations and functionalization are important to be able to harvest a functional cell sheet, to be further transplanted. Then, we present aspects of tissue mimics and three‐dimensional reconstruction of a tissue in vitro . Finally, we give an overview of clinical applications and clinically relevant animal experimentations of the technology, such as cardiomyopathy, visual acuity, periodonty, oesophageal ulcerations and type 1 diabetes.