Premium
Characterization of the expanded T‐cell populations in patients with Wegener's granulomatosis
Author(s) -
GISCOMBE R.,
WANG X. B.,
KAKOULIDOU M.,
LEFVERT A. K.
Publication year - 2006
Publication title -
journal of internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.625
H-Index - 160
eISSN - 1365-2796
pISSN - 0954-6820
DOI - 10.1111/j.1365-2796.2006.01688.x
Subject(s) - cd137 , cd8 , population , immunology , il 2 receptor , medicine , t cell , antigen , immune system , environmental health
. Objectives. Wegener's granulomatosis (WG) is a chronic inflammatory disease characterized by granulomatosis inflammation, systemic vasculitis and glomerulonephritis. In patients, the peripheral T cells are characterized by mono/oligoclonal CD4 + /CD8 + T‐cell AV/BV receptor expansions, with aberrant expression of activation markers. This study was designed to characterize the phenotypic differences between the expanded and nonexpanded T‐cell populations. Expression of markers for activation, costimulation and adhesion molecules was examined. As earlier studies have shown aberrant expression of CD28/CD152, we also analysed the expression of another costimulatory system, the tumour necrotic factor receptor (TNFR) superfamily proteins. Design. Fluorocrome‐conjugated monoclonal antibodies and flow cytometry was used to analyse the expression of the different markers on the surface of the expanded and nonexpanded subsets of T cells. Setting. The Karolinska Hospital and Karolinska Institutet in Stockholm, Sweden. Subjects. Nine patients with WG (six men and three women) had 16 TCRAV/BV CD4 + /CD8 + expanded populations that were characterized. Results. The expanded TCRA/BV CD4 + and CD8 + cells had lower percentages of cells expressing CD28 and higher of those expressing CD152 (CTLA‐4). The expanded CD4 + population had more cells expressing HLA‐DR, CD57 and CCR5 (CD195), whilst the expression of CD25 was present on fewer of the expanded cells. The expanded CD8 + population contained more cells expressing CD137 (4‐1BB), CD137 (4‐1BBL), CD30 (Ki‐1), CD40 and CD134 (OX40), Conclusions. There were marked differences in the phenotypes of expanded and nonexpanded T‐cell populations.