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How should we measure n‐3 polyunsaturated fatty acids and their metabolites in humans?
Author(s) -
FISCHER S.
Publication year - 1989
Publication title -
journal of internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.625
H-Index - 160
eISSN - 1365-2796
pISSN - 0954-6820
DOI - 10.1111/j.1365-2796.1989.tb01431.x
Subject(s) - polyunsaturated fatty acid , arachidonic acid , chromatography , mass spectrometry , gas chromatography , high performance liquid chromatography , fatty acid , radioimmunoassay , chemistry , biochemistry , enzyme
. Growing interest in nutritional intervention with n‐3 polyunsaturated fatty acids demands reliable analyses of these fatty acids and their corresponding eicosanoids in vivo or ex vivo . n‐3 polyunsaturated fatty acids are preferably assayed by capillary gas‐liquid chromatography after extraction of the lipid classes, their separation by chromatographic methods and conversion of the fatty acids to methylesters. n‐3 eicosanoids have to be separated from their arachidonic acid analogues by HPLC or by gas‐liquid chromatography and are then quantified by UV‐spectroscopy or mass‐spectrometry. Structure elucidation has to be performed by chemical reactions and by mass spectrometry. Antibodies for radioimmunoassays against n‐3 eicosanoids are not yet available.