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Cigarette smoke impairment of human lymphocyte function by inhibition of transglutaminase
Author(s) -
BERNTORP K.,
EKMAN M.,
BERNTORP E.
Publication year - 1989
Publication title -
journal of internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.625
H-Index - 160
eISSN - 1365-2796
pISSN - 0954-6820
DOI - 10.1111/j.1365-2796.1989.tb01358.x
Subject(s) - tissue transglutaminase , lymphocyte , in vitro , medicine , thymidine , tobacco smoke , in vivo , immunology , endocrinology , pharmacology , microbiology and biotechnology , biochemistry , chemistry , biology , enzyme , environmental health
Berntorp K, Ekman M, Berntorp E (Department of Internal Medicine. University of Lund, Malmö General Hospital, Malmö, Sweden). Cigarette smoke impairment of human lymphocyte function by inhibition of transglutaminase. The in‐vitro and in‐vivo effects of cigarette smoke were studied in human peripheral blood lymphocytes by applying a method for the capping of β 2 ‐microglobulin‐ or phytohaemagglutinin (PHA)‐stimulated lymphocyte transformation (measured as ( 3 H)thymidine incorporation) involving the transglutaminase pseudosubstrate monodansylthiacadaverine (MDTC), whose presence resulted in significantly reduced capping and ( 3 H)thymidine incorporation in a concentration‐dependent manner. The addition of dimethyl sulphoxide‐soluble particles from cigarette smoke to lymphocytes in vitro significantly reduced the capping ability and the PHA‐induced ( 3 H)thymidine incorporation. Whereas no significant change in MDTC‐dependent capping inhibition was seen in lymphocytes from smokers after 10 d abstinence from smoking, there was a marked decrease in ( 3 H)thymidine incorporation in lymphocytes from smokers after smoking three cigarettes following 10 h abstinence. The tentative conclusion is that exposure to cigarette smoke, or smoke extract, impairs MDTC‐dependent capping inhibition and PHA‐stimulated lymphocyte transformation by transglutaminase inhibition.