Premium
Inactivation of koi‐herpesvirus in water using bacteria isolated from carp intestines and carp habitats
Author(s) -
Yoshida N,
Sasaki Rk,
Kasai H,
Yoshimizu M
Publication year - 2013
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2012.01449.x
Subject(s) - carp , biology , aquaculture , bacteria , cyprinus , pathogen , effluent , common carp , outbreak , veterinary medicine , fishery , microbiology and biotechnology , fish <actinopterygii> , virology , environmental engineering , medicine , environmental science , genetics
Since its first outbreak in J apan in 2003, koi‐herpesvirus ( KHV ) remains a challenge to the carp C yprinus carpio L . breeding industry. In this study, inactivation of KHV in water from carp habitats (carp habitat water) was investigated with the aim of developing a model for rapidly inactivating the pathogen in aquaculture effluent. Experiments with live fish showed that, in carp habitat water, KHV lost its infectivity within 3 days. Indications were that inactivation of KHV was caused by the antagonistic activity of bacteria (anti‐ KHV bacteria) in the water from carp habitats. Carp habitat water and the intestinal contents of carp were therefore screened for anti‐ KHV bacteria. Of 581 bacterial isolates, 23 showed anti‐ KHV activity. An effluent treatment model for the disinfection of KHV in aquaculture effluent water using anti‐ KHV bacteria was developed and evaluated. The model showed a decrease in cumulative mortality and in the number of KHV genome copies in kidney tissue of fish injected with treated effluent compared with a positive control. It is thought that anti‐ KHV bacteria isolated from the intestinal contents of carp and from carp habitat water can be used to control KHV outbreaks.