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Isolation of bacterial probiotic candidates from the gastrointestinal tract of rainbow trout, O ncorhynchus mykiss ( W albaum), and screening for inhibitory activity against F lavobacterium psychrophilum
Author(s) -
Burbank D R,
LaPatra S E,
Fornshell G,
Cain K D
Publication year - 2012
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2012.01432.x
Subject(s) - rainbow trout , biology , probiotic , microbiology and biotechnology , gastrointestinal tract , bacteria , trout , in vitro , isolation (microbiology) , fish <actinopterygii> , biochemistry , fishery , genetics
In this study, 318 bacterial strains were isolated from the gastrointestinal ( GI ) tracts of 29 rainbow trout, O ncorhynchus mykiss ( W albaum). These bacteria were screened in vitro for their ability to inhibit growth of F lavobacterium psychrophilum , the causative agent of coldwater disease. Bacteria observed to inhibit F . psychrophilum growth were further screened against rainbow trout bile, as an indicator of their ability to survive in the GI tract. This screening resulted in narrowing the pool to 24 bacterial isolates. Those 24 isolates were then tested for pathogenicity in rainbow trout by intraperitoneal injection. Following a 28‐day challenge, eight isolates were shown to cause direct mortality and were eliminated from further study. As a result, 16 bacterial isolates were identified as probiotic candidates with the potential to control or reduce disease caused by F . psychrophilum .