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Enhancement of Hsp70 synthesis protects common carp, Cyprinus carpio L., against lethal ammonia toxicity
Author(s) -
Sung Y Y,
Roberts R J,
Bossier P
Publication year - 2012
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2012.01397.x
Subject(s) - cyprinus , biology , common carp , ammonia , hsp70 , carp , bioassay , toxicology , toxicity , zoology , fishery , fish <actinopterygii> , biochemistry , ecology , heat shock protein , medicine , gene
Exposure to TEX‐OE ® , a patented extract of the prickly pear cactus ( Opuntia ficus indica ) containing chaperone‐stimulating factor, was shown to protect common carp, Cyprinus carpio L., fingerlings against acute ammonia stress. Survival was enhanced twofold from 50% to 95% after exposure to 5.92 mg L −1 NH 3 , a level determined in the ammonia challenge bioassay as the 1‐h LD50 concentration for this species. Survival of TEX‐OE ® ‐pre‐exposed fish was enhanced by 20% over non‐exposed controls during lethal ammonia challenge (14.21 mg L −1 NH 3 ). Increase in the levels of gill and muscle Hsp70 was evident in TEX‐OE ® ‐pre‐exposed fish but not in the unexposed controls, indicating that application of TEX‐OE ® accelerated carp endogenous Hsp70 synthesis during ammonia perturbation. Protection against ammonia was correlated with Hsp70 accretion.