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Bacterial host interaction of GFP‐labelled Vibrio anguillarum HI‐610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae
Author(s) -
Rekecki A,
Gunasekara R A Y S A,
Dierckens K,
Laureau S,
Boon N,
Favoreel H,
Cornelissen M,
Sorgeloos P,
Ducatelle R,
Bossier P,
Van den Broeck W
Publication year - 2012
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2011.01342.x
Subject(s) - dicentrarchus , vibrio anguillarum , sea bass , biology , microbiology and biotechnology , hindgut , virulence , pathogen , bacteria , vibrio , larva , midgut , fishery , ecology , fish <actinopterygii> , biochemistry , gene , genetics
The location and cell damage caused by Vibrio anguillarum , the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V. anguillarum with sea bass larvae. In the present study, germ‐free sea bass larvae were orally exposed to a V. anguillarum HI‐610 pathogen labelled with the green fluorescent protein (GFP‐HI‐610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post‐exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid‐ and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.