Premium
Detection of antigenic proteins expressed by lymphocystis virus as vaccine candidates in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)
Author(s) -
Jang H B,
Kim Y R,
Cha I S,
Noh S W,
Park S B,
Ohtani M,
Hikima J,
Aoki T,
Jung T S
Publication year - 2011
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2011.01268.x
Subject(s) - olive flounder , biology , capsid , antiserum , paralichthys , antigen , virology , flounder , gel electrophoresis , microbiology and biotechnology , virulence , virus , fish <actinopterygii> , gene , fishery , immunology , biochemistry
Although the major capsid proteins (MCPs) of lymphocystis disease virus (LCDV) have been characterized, little is known about the host‐derived immune response to MCPs and other LCDV antigenic proteins. To identify antigenic proteins of LCDV that could be used as vaccine candidates in olive flounder, Paralichthys olivaceus , we analysed the viral proteins responsible for its virulence by applying immuno‐proteomics. LCDV proteins were separated by one‐dimensional gel electrophoresis, transferred to polyvinylidene difluoride membrane, and probed with homogeneous P. olivaceus antisera elicited by LCDV natural infection and vaccination with formalin‐killed LCDV. Four immune‐reactive proteins were obtained at 68‐, 51‐, 41‐ and 21 kDa using antisera collected from natural infection while two proteins at 51‐ and 21 kDa exhibited response to antisera from vaccinated fish, indicating that the latter two proteins have vaccine potential. Using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry and nanoelectrospray MS/MS, the 51 and 21 kDa proteins were identified as MCP and an unknown protein, respectively.